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. 2008 Dec 5;283(49):34384–34392. doi: 10.1074/jbc.M804707200

FIGURE 2.

FIGURE 2.

Mutations in TRP_2 disrupt channel activity. a, Western analysis of HEK293 cell lysates transfected for 24 h with either YFP alone (con) or YFP + full-length WT, K202A/R204A (KR), S209R/S213F (SS), or S209R (S) TRPC3 mutants blotted with anti-Myc antibody. b, quantification of the area under the curve for Sr2+ entry in the presence of 100 μm OAG or 100 μm CCH stimulation in HEK293 cells transfected with either YFP alone (con) or YFP + full-length WT, K202A/R204A, S209R/S213F, or S209R TRPC3 mutants (S.E., n = 3, *, p value <0.01, Student's t test) (see supplemental Fig. S1, a and b for traces). c, whole cell electrophysiological recordings in HEK293 cells transfected with either full-length WT (red) or SS (black) TRPC3 and stimulated with 100 μm OAG or 100 μm carbachol.