Mutations in TRP_2 disrupt channel activity.
a, Western
analysis of HEK293 cell lysates transfected for 24 h with either YFP alone
(con) or YFP + full-length WT, K202A/R204A (KR), S209R/S213F
(SS), or S209R (S) TRPC3 mutants blotted with anti-Myc
antibody. b, quantification of the area under the curve for
Sr2+ entry in the presence of 100 μm OAG or 100
μm CCH stimulation in HEK293 cells transfected with either YFP
alone (con) or YFP + full-length WT, K202A/R204A, S209R/S213F, or
S209R TRPC3 mutants (S.E., n = 3, *, p value
<0.01, Student's t test) (see supplemental Fig. S1, a and
b for traces). c, whole cell electrophysiological recordings
in HEK293 cells transfected with either full-length WT (red) or SS
(black) TRPC3 and stimulated with 100 μm OAG or 100
μm carbachol.