FIGURE 4.

Electron microscopy and quantification of vesicle mobility at the plasma membrane. a, transmission electron microscopy of HeLa cells transfected with either full-length Myc-tagged WT or SS TRPC3. Arrows depict TRPC3 immunoreactivity at the plasma membrane. Examples of blebbing are presented in the two right panels of SS TRPC3-Myc. b, graph demonstrating the rate of fluorescence recovery (FRAP) for HEK293 cells stably transfected with either YFP-tagged full-length WT (black) or SS (red) TRPC3. (S.D., n = 5, ^*, p value < 0.01 determined by Student's t test). c, graphs demonstrating the rate of fluorescence loss (FLIP) for cells transfected as in b. Left to right, total (cytosolic and membrane) ROI bleaching rate, cytosolic ROI bleaching rate, and membrane ROI bleaching rate. (S.D., n = 5, ^*, p value < 0.01 determined by Student's t test). For b and c, see“Materials and Methods,” and supplemental Fig. S1e.