FIGURE 2.

Characterization of laminin-332 purified from conditioned media of vector-, GnT-III-, or GnT-V-MKN45 transfectants. Purified laminin-332s, vector-Lm332 (vector), GnT-III-Lm332 (GnT-III), and GnT-V-Lm332 (GnT-V), were run on 4–7.5% gradient gels and 6% gels under nonreducing (–ME) and reducing conditions (+ME), respectively. After SDS-PAGE, separated proteins were stained with CBB (A; CBB) or transferred to the nitrocellulose membranes. Blotted proteins were probed with monoclonal antibodies against laminin α3 (Lsαc3), β3 (kalinin B1), and γ2 (D4B5) chain (B; I.B.). Ordinates indicate molecular sizes in kDa of marker proteins and laminin chains.