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Lectin blotting analysis of purified laminin-332s. 100 ng of laminin-332s (vector-, GnT-III-, and GnT-V-Lm332) was separated on 6% SDS-PAGE gel and then blotted onto the nitrocellulose membranes. Blotted proteins were probed with E4-PHA (top) and L4-PHA (bottom) lectin. Ordinates indicate molecular sizes in kDa of marker proteins and laminin chains.
