Skip to main content
. 2008 Nov 28;283(48):33036–33045. doi: 10.1074/jbc.M804526200

FIGURE 5.

FIGURE 5.

Cell adhesion activities of purified laminin-332s. A, cell adhesion activities of vector-Lm332 (crosses), GnT-III-Lm332 (open squares), and GnT-V-Lm332 (closed circles). The 96-well plates were coated with the indicated concentrations of each Lm332. After 1.2% BSA blocking, Lm332-null keratinocytes suspended in supplement-free keratinocyte medium were plated to each well and incubated for 20 min. Each point represents the mean of triplicate assays. Other experimental conditions are described in “Experimental Procedures.” B, 2 μg/ml Lm332s were coated to the 96-well ELISA plates at 4 °C overnight. After blocking those wells with 1.2% BSA, coating efficiency of three kinds of Lm332s was estimated by ELISA using anti-laminin γ2 chain antibody. C, inhibitory effects of function blocking antibody against integrin α3(anti-α3) or control mouse IgG (IgG) on cell adhesion of Lm332-null keratinocytes to different kinds of Lm332s. The numbers in parentheses indicate the concentrations for Lm332 (μg/ml). Each bar represents the mean ± S.D. of triplicate assays.