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. 2008 Nov 28;283(48):33110–33118. doi: 10.1074/jbc.M804255200

FIGURE 1.

FIGURE 1.

DOXO-induced cell death accompanied with BTG2/TIS21 expression. A, immunocytochemistry showing chromatin condensation only in the DOXO-treated cells. HeLa cells were treated with either DMSO or DOXO (1.0 μg/ml) for 24 h, and the nuclei were stained with Hoechst dye. B, immunoblot analysis revealing degradations of lamin A/C and PARP by DOXO treatment. HeLa cells were treated with indicated concentrations of DOXO for 24 h. Cleavages of PARP and lamin A/C (the substrates of caspase-3 and caspase-7, and caspase-6, respectively) were observed with 0.5 μg/ml and more of DOXO. α-Tubulin was examined as a loading control. C, RT-PCR analysis showing a higher expression of BTG2/TIS21 mRNA in 12 h of DOXO treatment. HeLa cells were exposed to 1.0 μg/ml of DOXO for indicated times. D, confirmations of time and concentration of DOXO treatment to induce HeLa cell death. HeLa cells were exposed to 1.0 μg/ml of DOXO for indicated times. Cleavage of lamin A/C was observed after 12 h of DOXO treatment by immunoblot analysis. The data represent typical results from four independent experiments. W.B., Western blot.