DOXO-induced cell death accompanied with BTG2/TIS21
expression. A, immunocytochemistry showing chromatin condensation
only in the DOXO-treated cells. HeLa cells were treated with either DMSO or
DOXO (1.0 μg/ml) for 24 h, and the nuclei were stained with Hoechst dye.
B, immunoblot analysis revealing degradations of lamin A/C and PARP
by DOXO treatment. HeLa cells were treated with indicated concentrations of
DOXO for 24 h. Cleavages of PARP and lamin A/C (the substrates of caspase-3
and caspase-7, and caspase-6, respectively) were observed with 0.5 μg/ml
and more of DOXO. α-Tubulin was examined as a loading control.
C, RT-PCR analysis showing a higher expression of
BTG2/TIS21 mRNA in 12 h of DOXO treatment. HeLa cells were exposed
to 1.0 μg/ml of DOXO for indicated times. D, confirmations of time
and concentration of DOXO treatment to induce HeLa cell death. HeLa cells were
exposed to 1.0 μg/ml of DOXO for indicated times. Cleavage of lamin A/C was
observed after 12 h of DOXO treatment by immunoblot analysis. The data
represent typical results from four independent experiments. W.B.,
Western blot.