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Shp-2 associates with TERT. HEK cells were transfected with TERT-myc and endogenous Shp-2 was immunoprecipitated from 250 μg of nuclear protein. Immunoblots with the precipitate (IP) and 25 μg of total protein from the supernatant (SN) were performed with an anti-Myc antibody (TERT-myc, upper panel) and anti-Shp-2 (lower panel). IgG served as negative control. The inset shows the reciprocal immunoprecipitation with an anti-Myc antibody.
