TABLE 2.
Inhibition of barium currents at the first test pulse (IP1) and increase in PF following agonist-mediated receptor activation Data are given as the mean ± S.E. and determined if significantly different from zero (one sample t test).
| Agonist | Receptor | n | IP1 inhibition | p value | PF increase | p value |
|---|---|---|---|---|---|---|
| % | % | |||||
| 50 nm NPY | WT-Y1 | 21 | 73.8 ± 3.6 | ***a | 94.7 ± 11.6 | *** |
| T6.30A-Y1 | 7 | 84.5 ± 4.1 | *** | 188.4 ± 65.0 | *b | |
| N6.34A-Y1 | 12 | 82.1 ± 2.5 | *** | 197.5 ± 56.0 | *** | |
| Y1-TM3-TM6-MOR | 4 | 7.8 ± 9.0 | nsc | N/A | N/A | |
| Y1-ICL3-MOR | 17 | 60.6 ± 2.8 | *** | 26.7 ± 9.5 | * | |
| Y1-ICL3(T6.34K)-MOR | 9 | 62.4 ± 1.3 | *** | 11.6 ± 6.9 | ns | |
| 1 μm DAMGO | WT-MOR | 4 | 61.2 ± 4.2 | *** | 34.4 ± 5.7 | **d |
| T6.34K-MOR | 4 | 36.0 ± 6.2 | ** | –13.3 ± 9.8 | ns |
a***
, p < 0.0001
b*
, p < 0.05
c
ns, not significant; one-sample t test
d**
, p < 0.01