FIGURE 3.

hFis1 overexpression reduces cellular ATP content and increases lactate production. After infection with adenovirus coding for hFis1, DN-Mfn1, or WT-Mfn1, INS-1E cells were incubated for 48 h with or without doxycycline (500 ng/ml). A, ATP contents were measured 10 min after stimulation with 2.8 mm (basal) or 16.5 mm glucose. Cellular ATP was normalized to the DNA content and expressed as the percentage of control for each group (basal level in non-induced cells; n = 7). B, lactate production after a 10-min incubation with either 5.5 mm or 16.5 mm glucose was normalized to the DNA content (pmol of lactate/μg of DNA (n = 6)). Data are presented as means ± S.E. ^* and ^** denote p < 0.05 and p < 0.01, respectively.