Southern hybridization of CYP121 delinquents. Genomic DNA
was digested with BamHI and hybridized with the CYP121 probe. The WT
(2.5 kb), integrated (4.2 kb), and deletion (1.3 kb) bands are indicated.
Lane 1, 1-kb ladder; lane 2, WT; lanes 3–7,
CYP121 delinquents; lane 8, 1-kb ladder. In lanes
3–7, the upper band reports the presence of the newly integrated
CYP121 gene, whereas the lower band reports on the probe
hybridization to the remnant of the original chromosomal copy of
CYP121 following generation of the in-frame deletion.