FIGURE 5.

X-ray scattering profiles of the Pelle-Tube-dMyD88 death domain heterotrimer (a) and the Tube-dMyD88 death domain heterodimer (b) are shown (S=2 Sin(θ)/λ, where θ is the scattering angle and λ the wavelength 0.154 nm). The inset shows the distance distribution functions for both complexes from which maximum particle dimensions of 9.2 (heterotrimer) and 8.0 nm (heterodimer) are obtained. Two orthogonal orientations of the ab initio models of the heterotrimeric (c) and the heterodimeric (d) complex are shown. The superimposition of the SAXS-derived model of the Pelle-Tube-dMyD88 heterotrimer with the high resolution x-ray structure of the Pelle-Tube death domain heterodimer (e) is shown. The Tube death domain is colored red.