FIGURE 2.

Prdx6 hyperoxidation is dependent on the concentration of H₂O₂. HeLa (A) and HEK293 (B) cells were treated with different concentrations of H₂O₂, as indicated, for 20 min. Cell lysates were subjected to immunoblotting with an anti-Prdxs-SO₃H antibody that recognizes Prdx1-, Prdx2-, and Prdx3-SO₃H, or with anti-Prdx6 SO₃H, anti-Prdx1, anit-Prdx2, anti-Prdx3, anti-Prdx6, or anti-GAPDH antibodies. Each graph (left) represents the band intensity quantified by densitometric analysis (ImageJ 1.40 software). HeLa (C) and HEK293 (D) cells were treated with different concentrations of H₂O₂, as indicated, for different amounts of time. Cell lysates were subjected to immunoblotting as described in A and B.