TABLE 1.
FRET distances and corresponding dwell times for the FRET pairs a-EGFP versus ε56 and a-EGFP versus γ106 on F0F1-ATP synthase during ATP hydrolysis
The shortest dwell times are highlighted (bold); dwell times are given with errors (± σ S.D.) for the fitting. Labeling γ106 slightly reduced the catalytic activities as reported previously (11, 33). Distances between fluorophore positions I, II, and III (or I′, II′, and III′ for γ106, respectively) and C refer to positions denoted in Fig. 5.
| Distance | Dwell time | |
|---|---|---|
| nm | ms | |
| Alexa568 at ε56 | ||
| Low FRET (I ↔ C) | 6.8 | 9 ± 1 |
| Medium FRET (II ↔ C) | 5.4 | 11 ± 1 |
| High FRET (III ↔ C) | 4.0 | 10 ± 1 |
| Alexa568 at γ106 | ||
| Low FRET (I′ ↔ C) | 7.6 | 20.3 ± 3 |
| Medium FRET (II′ ↔ C) | 5.2 | 20.2 ± 1 |
| High FRET (III′ ↔ C) | 3.3 | 16.0 ± 1 |