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FRL2N inhibits actin bundling by FRL3.FH2 and FRL2.FH2.2+3. FRL2N (0, 0.1, and 0.5 μm) was incubated with F-actin (2 μm) (A) and FRL2.FH2 (0.5 μm) (B), FRL3.FH2 (0.5 μm) (C), or FRL2.2+3 (0.5 μm) (D) and then centrifuged at 16,000 × g for 10 min to pellet F-actin bundles. Equivalent samples of the supernatant and pellet were subjected to SDS-PAGE, and the proteins were visualized with Coomassie Blue. The arrow indicates the FRL2N protein band.
