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. 2008 Nov 28;283(48):33750–33762. doi: 10.1074/jbc.M803156200

FIGURE 3.

FIGURE 3.

FRL2 contains a functional DID but not a functional DAD. A and B, FRL3.F1F2 (red) was coexpressed with FRL2N or FRL3N (white) by transient transfection. Co-expression of either FRL2N or FRL3N (1.2 μg of DNA transfected) inhibits FRL3.F1F2 (0.3 μg of DNA)-induced stress fiber formation (green, 98%, n = 103) (compare cells in A and B with Fig. 1C). C and D, FRL2.F1F2 (red) was co-expressed with FRL2N or FRL3N (white) by transient transfection. Co-expression of either FRL2N or FRL3N did not inhibit FRL2.F1F2-induced stress fiber formation (green) (97%, n = 100). E, expression of FRL2N or FRL3N (1 μg of DNA transfected) inhibits FRL3.F1F2 (0.1 μg of DNA)-induced activation of an SRF reporter gene but has no effect on FRL2.F1F2-induced SRF activation. Reporter gene activation in the absence of inhibitor was standardized to 100%. Error bars, S.E., n = 3.