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. 2008 Nov 28;283(48):33750–33762. doi: 10.1074/jbc.M803156200

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Copyright © 2008, The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — C-terminal derivatives of FRL2 and FRL3 bind to N-terminal FRL2 and FRL3 derivatives in a DAD-dependent manner. A, FLAG-tagged FRL3N (1.5 μg of DNA) was co-expressed in NIH 3T3 cells with Myc-tagged FRL3.FH2 or FH2.1045 and FRL2.F1F2, F1F2.2+3, or F1F2.986 (1.5 μg of DNA). The FLAG-tagged protein efficiently co-immunoprecipitated (IP) FRL3.FH2, FRL2.F1F2, and FRL2.F1F2.2+3 but not FRL3.FH2.1045 or FRL2.F1F2.986 (compare lanes 1 and 2 with lanes 3 and 4 and lanes 5-8 with lanes 9 and 10). B, FLAG-tagged FRL2N was co-expressed in NIH 3T3 cells with Myc-tagged FRL3.FH2 or FH2.1045 and FRL2.F1F2, F1F2.2+3, or F1F2.986. The FLAG-tagged protein efficiently co-immunoprecipitated FRL3.FH2, FRL2.F1F2, and FRL2.F1F2.2+3 but not FRL3.FH2.1045 or FRL2.F1F2.986, which lack the most distal DAD motif (compare lanes 1 and 2 with lanes 3 and 4 and lanes 5-8 with lanes 9 and 10).