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. 2008 Nov 28;283(48):33750–33762. doi: 10.1074/jbc.M803156200

FIGURE 8.

FIGURE 8.

Deletion of the C-terminal DAD motifs reduces FRL3.FH2 activity in vivo. A, as in Fig. 1, expression of FRL3.FH2 induces thick stress fibers, actin aggregates, and peripheral F-actin accumulation (98%, n = 101). B, FRL3.FH2.1045 behaves similarly to FH2 (83%, n = 100). C, FRL3.FH2.1023 induces thick stress fibers (75%, n = 102) but no actin aggregates or peripheral F-actin. D, deletion of the distal and proximal DAD motifs reduces the ability of FRL3.FH2 to induce activation of an SRF reporter gene. Inset, relative levels of expression of FRL3.FH2, FRL3.FH2.1045, and FRL3.FH2.1023 as determined by immunoblotting. Reporter gene activity was standardized to activation induced by expression of an SRF-VP16 control fusion protein. Error bars, S.E., n = 3. E, schematic of FRL3.FH2 derivatives.