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. 2008 Nov 28;283(48):33750–33762. doi: 10.1074/jbc.M803156200

FIGURE 9.

FIGURE 9.

Deletion of the C-terminal DAD modifies FRL2.FH2 activity in vivo. A, as in Fig. 2, expression of FRL2.FH2 induces thick stress fibers, actin aggregates, and peripheral F-actin accumulation (97%, n = 100). B, FRL2.FH2.986 behaves similarly to FH2 (99%, n = 100). C, FRL2.FH2.965 induces fewer actin aggregates and peripheral F-actin (88%, n=100). D, FRL2.FH2.2+3 behaves similarly to FRL2.FH2(98%, n = 100). E, deletion of both distal and proximal DAD motifs does not affect FRL2.FH2-induced SRF reporter gene activation. Inset, relative levels of expression of FRL2.FH2, FRL2.FH2.986, FRL2.FH2.965, and FRL2.FH2.2+3, as determined by immunoblotting. Reporter gene activity was standardized to activation induced by expression of an SRF-VP16 control fusion protein. Error bars, S.E., n = 3. F, schematic of FRL2.FH2 derivatives.