FIGURE 10.

Effect of LAD and HAD on Src activation in vivo. a, tibialis muscle homogenates from 22 weeks (w) LAD- or HAD-fed mice were separated by SDS-PAGE followed by immunoblotting with antibodies to phosphotyrosine 416 Src or total Src. The autoradiographs shown are representative of three independent experiments. b, densitometric analysis of phosphotyrosine 416 Src normalized on total Src. c, tibialis muscle homogenates from 9-week LAD- or HAD-fed mice were separated by SDS-PAGE followed by immunoblotting with antibodies to phosphotyrosine 416 Src or total Src. The autoradiographs shown are representative of three independent experiments. d, densitometric analysis of phosphotyrosine 416 Src normalized on total Src. *, statistically significant differences (*, p < 0.05).