FIGURE 12.

Effect of glycated albumin on PKCα and Src activation in vivo. 4-Week-old female mice of the C57/BL6 strain were injected with 50 mg/kg of HGA or HA or PBS either directly in the quadriceps (GA-Im: a, b, c, and d) or in the tail vein (GA-Iv: e, f, g, and h) as described under “Experimental Procedures. a and c, muscle tissue lysates from muscle-injected mice were blotted with anti-phospho PKCα, and PKCα phosphorylation was analyzed by densitometry. b and d, muscle tissue lysates from muscle-injected mice were blotted with anti-phospho Src Tyr⁴¹⁶ antibody and Src phosphorylation was analyzed by densitometry. e and g, muscle tissue lysates from tail vein-injected mice were blotted with anti-phospho PKCα, and PKCα phosphorylation was analyzed by densitometry. f and h, muscle tissue lysates from tail vein-injected mice were blotted with anti-phospho-Src Tyr⁴¹⁶ antibody, and Src phosphorylation was analyzed by densitometry. A representative autoradiograph is shown. Bars represent the mean ± S.D. of three different experiments. *, statistically significant differences (*, p < 0.05).