FIGURE 2.

sRAGE abrogates HGA-activated Src and PKCα activation in L6 skeletal muscle cells. a, L6 skeletal muscle cells were incubated with 0.1 mg/ml HA or HGA for 24 h in the absence or in the presence of 50 μg/ml sRAGE. PKCα activity was assayed in the immunoprecipitates as described under “Experimental Procedures.” Bars represent the mean ± S.D. of three independent experiments. b, L6 skeletal muscle cells were incubated with 0.1 mg/ml HA or HGA for 24 h in the presence or in the absence of 50 μg/ml sRAGE, lysed as described under “Experimental Procedures”; lysates were then separated by SDS-PAGE followed by immunoblotting with antibodies to phosphotyrosine 416 Src or total Src. The autoradiographs shown are representative of three independent experiments. *, statistically significant differences (**, p < 0.01; ***, p < 0.001).