FIGURE 5.

HGA-induced association of PKCα, Src, and RAGE in L6 skeletal muscle cells. a, lysates from L6 skeletal muscle cells treated or not with 0.1 mg/ml HA or HGA for 24 h were precipitated (IP) with preimmune serum (PI) or with selective anti-RAGE antibodies and immunoblotted with anti-PKCα, anti-Src, or anti-RAGE antibodies. The autoradiographs shown are representative of three independent experiments. b, lysates from L6 skeletal muscle cells treated as above were precipitated with selective anti-RAGE antibodies and immunoblotted with PKCβ, PKCδ, PKCζ, or PKCα antibodies. The autoradiographs shown are representative of three independent experiments. c, L6 skeletal muscle cells were treated with 0.1 mg/ml HA or HGA for the indicated times. Cells were lysed, cell lysates were then precipitated with selective anti-RAGE antibodies, and PKC activity was measured as described under “Experimental Procedures.” Bars represent the mean ± S.D. of values obtained from three independent experiments in duplicate. *, statistically significant differences (***, p < 0.001).