FIGURE 9.

Effect of LAD and HAD on PKB/Akt and PKCα activation in vivo. a, 4-week-old female mice of the C57/BL6 strain were fed a LAD or a HAD diet as described under “Experimental Procedures.” At week (w) 22 (a) and week 9 (b), mice were injected intraperitoneally with insulin (0.125 milliunits/g of body weight), and muscle tissues were collected and homogenized. Tissue homogenates were subjected to SDS-PAGE followed by immunoblotting with antibodies to phosphoserine 473 PKB/Akt and to PKB/Akt as a control. PKB/Akt phosphorylation was analyzed by densitometry. Bars represent the mean ± S.D. of three different experiments. c, at week 9 or at week 22 muscle tissues homogenates were subjected to immunoprecipitation with PKCα antibodies, and specific PKCα activity was measured as described under “Experimental Procedures.” Bars represent the mean ± S.D. of three independent experiments. *, statistically significant differences (**, p < 0.01; ***, p < 0.001).