Effect of LAD and HAD on PKB/Akt and PKCα activation in
vivo. a, 4-week-old female mice of the C57/BL6 strain were
fed a LAD or a HAD diet as described under “Experimental
Procedures.” At week (w) 22 (a) and week 9
(b), mice were injected intraperitoneally with insulin (0.125
milliunits/g of body weight), and muscle tissues were collected and
homogenized. Tissue homogenates were subjected to SDS-PAGE followed by
immunoblotting with antibodies to phosphoserine 473 PKB/Akt and to PKB/Akt as
a control. PKB/Akt phosphorylation was analyzed by densitometry. Bars
represent the mean ± S.D. of three different experiments. c,
at week 9 or at week 22 muscle tissues homogenates were subjected to
immunoprecipitation with PKCα antibodies, and specific PKCα
activity was measured as described under “Experimental
Procedures.” Bars represent the mean ± S.D. of three
independent experiments. *, statistically significant differences (**,
p < 0.01; ***, p < 0.001).