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. 2008 Dec 26;283(52):36100–36107. doi: 10.1074/jbc.M804438200

FIGURE 1.

FIGURE 1.

SDS-PAGE analysis of the digestion patterns of deseasin MCP-01 on fish- and bovine-insoluble collagens and the N-terminal sequences of some peptides released from bovine-insoluble type I collagens. A, digestion result of fish insoluble collagen fiber from Pseudosciaena polyactis by MCP-01. Lane 1, the result of 5 mg of fish-insoluble collagen fiber incubated with 18 μg of MCP-01 at 60 °C for 5 h; lane 2, control, the result of fish-insoluble collagen fiber incubated in 50 mm Tris-HCl without MCP-01 at 60 °C for 5 h. B, digestion result of bovine-insoluble type I collagen fiber by MCP-01. Lane 1, MCP-01 (18 μg) incubated in 50 mm Tris-HCl without collagen at 60 °C for 5 h; lane 2, 5 mg of bovine-insoluble type I collagen fiber incubated in 50 mm Tris-HCl without MCP-01 at 60 °C for 5 h; lanes 3–9, the digestion results of 5 mg of bovine insoluble type I collagen fiber incubated with 18 μg of MCP-01 at 60 °C for 5, 10, 15, 20, 30, 60, and 300 min, respectively. The N-terminal sequences of 6 released peptides are indicated on the right side. They were determined by Edman degradation using PROCISE491.