FIGURE 3.

Effect of temperature (A) and pH (B) on the collagenolytic activity of deseasin MCP-01. A, deseasin MCP-01 (▪) and the collagenase from C. histolyticum (○) was incubated with 5 mg of bovine-insoluble type I collagen fiber in 50 mm Tris-HCl (0.36 mm CaCl₂ added, pH 7.5) at various temperatures for 5 h, respectively. Collagenase activity was determined with the method described under “Experimental Procedures.” The enzyme activity of deseasin MCP-01 at 60 °C and that of the collagenase from C. histolyticum at 50 °C were taken as 100%. B, the collagenolytic activities of deseasin MCP-01 in 50 mm Tris-HCl from pH 6.0 to 10.0 were determined as described under “Experimental Procedures.” The highest activity was taken as 100%. The data are the mean of three experimental repeats with S.D. ≤ 5%.