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. 2008 Dec 26;283(52):36100–36107. doi: 10.1074/jbc.M804438200

FIGURE 5.

FIGURE 5.

A, binding ability of PKD domain and its mutants to bovine-insoluble type I collagen fiber and elastin. B, purified EGFP and fusion proteins of PKD and its three mutants. Various concentrations of EGFP, and fusion proteins of PKD and its mutants were incubated with a fixed amount of collagen (or elastin) in double distilled water at 40 °C for 1 h with mixing. The free fluorescence intensity in the solution before and after incubation was determined with spectrofluorometer. EGFP was used as the control. The figure shows the equilibrium adsorption isotherms (B versus F) by measuring the bounded protein amount (B) and the unbounded protein amount (F). The data represent the mean of three experimental repeats with S.D. ≤ 5%. ▪, PKD incubated with collagen; •, W34A incubated with collagen; ▴, W36A incubated with collagen; ▾, W34A/W36A incubated with collagen; ♦, EGFP incubated with collagen; □, PKD incubated with elastin.