TABLE 1.
The substrate specificity of deseasin MCP-01 compared with that of the collagenase from C. histolyticum
Note, the activities of MCP-01 and the collagenase from C. histolyticum to various substrates at 40 °C were measured with the methods described under “Experimental Procedures.” The data represent the mean of three experimental repeats with S.D. ≤ 5%.
| Substrate | MCP-01 | Collagenasea | |
|---|---|---|---|
| units/mg | |||
| Fish-insoluble collagen fiber | 10434 | 4456 | |
| Bovine-insoluble type I collagen fiber | 1608 | 5274 | |
| Bovine acid-dissolved type I collagen | 136.7 | 113.1 | |
| Bovine type II collagen | 67.6 | 157.8 | |
| Bovine type IV collagen | 89.3 | 97.0 | |
| Gelatin | 151.0 | 181.5 | |
| Casein | 0.68b | 0 | |
| Pz-PLGPRc | 24.8 | 24.6 | |
| Su-AAAd | 2.94b | 0 | |
a
The collagenase from C. histolyticum purchased from Worthington Biochemical Corp.
b
Data from our previous work [7].
c
4-Phenylazobenzyloxycarbonyl-Pro-Leu-Gly-Pro-Arg.
d
N-Succinyl-Ala-Ala-Ala-p-nitroanilide.