TABLE 2.

Effects of metal ions and protease inhibitors on the collagenolytic activity of deseasin MCP-01 to bovine-insoluble type I collagen fiber

Note, the enzyme activity of MCP-01 in buffer A without any metal ion or inhibitor was taken as control (100%). The data represent the mean of three experimental repeats with S.D. ≤ 5%.

Metal ion	Relative activity (%)		Metal ion	Relative activity (%)		Inhibitors	Residual activity (%)
	2 mm	4 mm		2 mm	4 mm		1 mm	5 mm
Control	100	100	Mn2+	85.7	89.3	Control	100	100
Li+	86.2	94.5	Fe2+	51.4	44.6	PMSFa	9.0	3.9
K+	100.4	100.2	Co2+	71.8	61.3	EDTA	7.9	11.6
Mg2+	79.4	79.9	Ni2+	40.8	31.2	EGTA	26.4	58.9
Ca2+	249.4	274.1	Zn2+	31.2	24.1	SC	43.6	28.8
Sr2+	92.2	98.7	Cu2+	45.2	37.4	o-P	69.3	63.4
Ba2+	90.3	82.6	Sn2+	99.1	88.9

^aPMSF, phenylmethylsulfonyl fluoride; SC, sodium citrate; o-P, o-phenanthroline.