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Primers used for cloning and mutagenesis of SusB NdeI and XhoI sites are underlined.
| Compounds | Primers |
|---|---|
| Wild type | Forward, ATTGCGCATATGCAACAGAAATTAACCTCA |
| Reverse, TTTTCCCTCGAGTTATAATCTTTTCAA | |
| E508Q | Forward, ATGGTGAATGCACACCAAGCAACCCGCCCTACC |
| Reverse, GGTAGGGCGGGTTGCTTGGTGTGCATTCACCAT | |
| E532Q | Forward, TCCGCCCGCGGTACACAATATGAATCATTCGGA |
| Reverse, TCCGAATGATTCATATTGTGTACCGCGGGCGGA | |
| E439Q | Forward, ATGATGATGCATCACCAAACTTCCGCTTCTGTA |
| Reverse, TACAGAAGCGGAAGTTTGGTGATGCATCATCAT |
