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. 2008 Dec 26;283(52):36532–36541. doi: 10.1074/jbc.M807144200

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Copyright © 2008, The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 7. — Trp fluorescence quenching studies of Rv3868 and NT-Rv3868 by acrylamide and ATP. The quenching of intrinsic fluorescence of the proteins was followed after the addition of increasing concentrations of acrylamide or ATP. A, fluorescence quenching observed in Rv3868 (•), Rv3868 + ATP (▾), and NT-Rv3868 (▴) proteins upon the addition of acrylamide. The Stern-Volmer quenching constants (K_SV) corresponded to plot slopes (Equation 6). B, relative decrease in TRP fluorescence upon the addition of ATP in Rv3868 + ATP + MgCl₂ (1), Rv3868 + ATP (2), and NT-Rv3868 + ATP+MgCl₂ (3) samples, respectively.