TABLE 3.
Phosphorylation status of recombinant MurC as determined by mass spectrometry
| Phosphorylated tryptic and chymotryptic peptide sequence | Number of detected phosphate groups | Phosphorylated residue(s) |
|---|---|---|
| LC-ESI/MS/MS | ||
| [348-372] FNGAAITDDYAHHPTEVTAVLSAAR | 1 | Thr362 |
| [348-372] FNGAAITDDYAHHPTEVTAVLSAAR | 1 | Thr365 |
| [348-372] FNGAAITDDYAHHPTEVTAVLSAAR | 2 | Thr362 and Thr365 |
| [40-56] TVTGSDAKDSRTLLPLR | 1 | Thr51 |
| [48-56] DSRTLLPLR | 1 | Thr51 |
| [108-130] RSDLLGELLEGSTQVLIAGTHGK | 1 | Thr120 |
| [109-130] SDLLGELLEGSTQVLIAGTHGK | 1 | Thr120 |
| [159-184] AGTNAHHGTGEVFIAEADESDASLLR | 1 | Thr167 |
| [131-158] TSTTSMSVVAMQAAGMDPSFAIGGQLNK | 1 | Thr133 |
| [124-141] IAGTHGKTSTTSMSVVAM | 1 | Thr133 |