FIGURE 2.

Distribution of iron in bacterioferredoxin-proficient and -deficient cells supplied with ⁵⁹Fe-chrysobactin. Native PAGE analysis of protein extracts was performed as described in Fig. 1. A, ⁵⁹Fe-chrysobactin was supplied at the concentration of 0.25 μm for the indicated times. B, ⁵⁹Fe-chrysobactin was supplied at the concentration of 0.5 μm for 40 min as indicated. The cells were then washed and grown for the indicated times (details are under “Experimental Procedures”); optical density at 600 nm (OD₆₀₀) of bacterial cultures is indicated. The strains are designated as in Fig. 1. Plasmid pAB14 contains the bfd gene under the control of its own promoter.