FIGURE 3.

Growth and transport characteristics of parental and relevant mutant strains treated with ⁵⁹Fe-chrysobactin. A, growth of bacterial cultures with (solid circles) or without (open circles) the addition of 1 μm ⁵⁹Fe-chrysobactin. ⁵⁹Fe-chrysobactin was added when A₆₀₀ = 0.4. Genotypes are shown on each graph. Growth curves of the sufD and sufE mutants (not shown) were similar to those obtained for the sufB and sufS mutants, respectively. The experiments were performed five times, and the data reported are the means of three independent experiments with standard deviation corresponding to less than 5%. B, amounts of iron (× 10⁶ atoms) incorporated into parental and relevant mutant cells after treatment with 0.25 μm ⁵⁹Fe-chrysobactin for 40 min. Transport assays were performed as described under “Experimental Procedures.” The data are the means of measurements obtained in three separate experiments, with error bars representing S.D.