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Quenching of intrinsic tryptophan fluorescence of the wild-type GlLeuRS (▪) and its CP2 deletion mutant (•) through tRNALeu titration. The concentration of tRNA was varied from 0 to 3 μm. The fluorescence intensity is given as an arbitrary unit, and the emission at 338 nm was quantified. Analysis of the wild-type GlLeuRS and its CP2 deletion mutant fluorescence intensity change versus tRNA concentration gave the KD values of 0.19 ± 0.02 μm and 0.37 ± 0.02 μm, respectively.
