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. 2008 Dec 26;283(52):36608–36616. doi: 10.1074/jbc.M806745200

FIGURE 4.

FIGURE 4.

Enzymatic activities of wild-type GlLeuRS and its mutants in the alanine scanning assay. A, the ATP-PPi exchange reaction was carried out using 1 mm Leu and 20 nm enzyme. B, the aminoacylation assay was carried out using 50 nm GlLeuRS or its mutants. C, post-transfer editing was performed using 2 μm [3H]Ile-tRNALeu from GltRNALeu and 20 nm GlLeuRS or its mutants. Symbols are GlLeuRS (•), GlLeuRS-Y581A (○), GlLeuRS-W586A (▾), GlLeuRS-K587A (▵), GlLeuRS-D588A (▪), GlLeuRS-D603A (□), GlLeuRS-K606A (♦), and GlLeuRS-F609A (⋄). Control (spontaneous hydrolysis) (▴) was performed in the absence of enzyme.