TABLE 1.
Relative kcat/Km of various mutants from G/LeuRS in ATP-PPi exchange reaction
| Substrate | GlLeuRS | K587A | D588A | D603A | K606R | K606E | K606L | K606D | |
|---|---|---|---|---|---|---|---|---|---|
| kcat/Km | |||||||||
| ATP | 1 | 0.43 | 0.57 | 0.91 | 0.98 | 0.90 | 0.85 | 0.86 | |
| Leu | 1 | 0.42 | 0.37 | 0.94 | 0.99 | 0.99 | 0.98 | 0.83 | |
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