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Heterologous expression of the candidate gene from F. graminearum in P. pastoris resulted in the formation of Δ3(E)-unsaturated fatty acids. Fatty acids from GlcCer isolated from P. pastoris transformants were analyzed by GLC as TMS-FAMEs. Δ3Fg converted 2-hydroxyoctadecanoic acid 18:0(2-OH), efficiently (50-100%) into 2-hydroxyoctadecenoic acid, 18:1(2-OH). FID, flame ionization detector; wt, wild type.
