Figure 2.
An activated form of STAT5 prevents AMPA-induced OLG cell death. A, Primary cultures of OLGs were transduced with an adenovirus expressing a constitutively activated STAT5A and GFP (STAT5Aca/GFP). B, Western blot analysis of phospho-STAT5 and bcl--XL in the cultured OLGs transduced with adenoviruses expressing GFP only or STAT5Aca with or without AMPA treatment for 24 h. A representative blot selected from three independent experiments was shown here. C, OLGs transduced with 107 ifu/ml of adenoviruses expressing GFP only or STAT4ca for 36 h and then treated with AMPA for 24 h. The cell survival was determined by MTT (n = 3). D, Bcl-xL protects AMPA-induced cell death in OLGs. The OLGs transduced with an adenovirus expressing bcl--XL (107 ifu/ml) for 36 h and then treated with AMPA for 24 h. GFP-positive cells were counted in control EGFP and Bcl-xL transduced cells (n = 3). The single asterisk denotes significant p < 0.05.