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. 2006 Apr 21;97(7):589–596. doi: 10.1111/j.1349-7006.2006.00225.x

Figure 2.

Figure 2

Analysis of the growth‐inhibitory effect of mouse PERIOD2 (mPER2) overexpression on (A–C) Lewis lung carcinoma cells and (D–F) EMT6 cells. (A,D) Colony formation after gene transfection. mPER2 suppressed colony formation dramatically. The colony‐forming efficiency of mPER2‐overproducing cells was significantly lower than control cells in three independent experiments (P < 0.01). (B,E) Cell proliferation assay to quantify the number of viable cells. The numbers under the horizontal axis indicate the days for cell culture. The results indicate a significant difference in cell growth speed between the two groups (P < 0.01). (C,F) Growth‐inhibitory effect of mPER2 on tumor cells detected by MTT assay. Absorbance at 490 nm showed a significant decrease in the growth of mPER2‐overproducing cells compared with vector control cells (P < 0.01).