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. Author manuscript; available in PMC: 2009 Mar 29.
Published in final edited form as: J Am Chem Soc. 2006 Nov 1;128(43):14081–14092. doi: 10.1021/ja0632207

Figure 4.

Figure 4

Spectral properties of 30 μM SNAFR-1 in 50 mM phosphate buffer at various pH values with 0.25% DMSO. The trend of spectral changes is indicated by arrows as the pH increases from 7 to 10. A = anionic form; N = neutral form. (A) Absorption spectra is shown; the * indicates the position of isosbestic points. (B–D) Excitation spectra is shown with emission monitored at 400, 540, and 620 nm, respectively. (E–H) Emission spectra excited at 325, 488, 514, and 543 nm, respectively, corresponding to common laser lines is shown. The legends for panels A–H are shown in panel H. All fluorescence spectra are normalized to the maximum of each data set.