Table 1.
protein mixture component | migration time, min | peak area | number of theoretical plates (N) × 105 |
---|---|---|---|
monomer (I) | 1.92 (0.31) | 220.54 (3.90) | 8.70 (3.24) |
dimer (II) | 1.75 (0.42) | 63.76 (4.38) | 5.75 (5.93) |
trimer (III) | 1.63 (0.45) | 14.20 (13.98) | 8.53 (12.77) |
Average migration time, peak area, and number of theoretical plates (N) values calculated for successive runs (n = 5) without regeneration of the PDADMAC coating. Percent relative standard deviation (% RSD) values are given in parentheses. Separations performed using 8 kV applied voltage.