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HPLC traces showing separation of phosphorothioate dinucleotide diastereomers (Rp, Sp) and phosphate dinucleotide (TpT). The untreated mixture is shown in black, and enzyme-treated mixtures are shown in red (nuclease P1) and green (NPP). Detection is by absorbance at 260 nm. Diastereomer assignments were made based on nuclease P1 selectivity and HPLC retention times (18).
