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. Author manuscript; available in PMC: 2010 Mar 1.
Published in final edited form as: Microbiology (Reading). 2009 Mar;155(Pt 3):882–890. doi: 10.1099/mic.0.023218-0

Fig. 5.

Fig. 5

Infection of amoebae by an srnA proA double mutant of L. pneumophila. H. vermiformis was infected with wild-type strain 130b (■), srnA mutant NU328 (○), proA mutant AA200 (◆), srnA proA double mutant NU335 (◇), and lspF mutant NU275 (□). At various times, the numbers of bacteria per well were determined. The values are the means (±SD) obtained from four wells, and are representative of two independent experiments. The numbers of each of the mutants recovered from the wells were significantly less than the numbers of wild-type, at 48 and 72 h post-inoculation (P<0.05; Student's t test). The numbers of the srnA proA double mutant were also significantly less than those of the proA mutant at 48 h, and the srnA mutant at 48 and 72 h, while being significantly more than those of the lspF mutant at 48 and 72 h (P<0.05). In a separate experiment, the numbers of the srnA proA double mutant recovered were significantly less than those of the proA mutant at 72 h.