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. Author manuscript; available in PMC: 2009 Mar 30.
Published in final edited form as: Eur J Immunol. 2008 Dec;38(12):3304–3315. doi: 10.1002/eji.200838602

Figure 3. Purified CD11b+ DC stimulate cell division by naïve F5 cells in vitro.

Figure 3

A) CD11c+ cells were purified from the MLN and ILN 8d after E61-13-H17 infection. CD11c+ cells were further subdivided using anti-CD11b and anti-CD8 antibodies and sterile FACS sorting. Naïve CFSE-labeled F5 cells were cultured (2×104 cells/well) with 5u/ml rIL-2 and sorted APCs (3–5×104 cells/well) in vitro for 4 days as shown. Gated populations of live CD45.1+ CD8 T cells are shown. The F5 peptide (1ug/ml) was used as a positive control for cell division. Three independent experiments gave very similar results.

B) Different subsets of CD11c+ cells were purified 30d p.i. with E61-13-H17 and cultured (3–5×104 cells/well) with naïve CFSE-labeled F5 cells (2×104 cells/well) 5u/ml rIL-2 in vitro for 4 days as shown. Gated populations of live CD45.1+ F5 cells analyzed for CFSE and PD-1 expression are shown. Three independent experiments gave very similar results.