Figure 2.

p53 binding site-dependent activation of the PTGF-β promoter by wild-type p53, but not by most p53 mutants. (A) Diagrammatic presentation of four luciferase reporter constructs driven by the PTGF-β promoter, containing, respectively, two (W/p53BS), the first one (W/Fp53BS), the second one (W/Fp53BS), and none of p53 binding sites (W/Op53BS). (B) Activation of luciferase activity in p53-binding site dependent manner. (C) Lack of transactivation of PTGF-β promoter by most p53 mutants. Four constructs were transiently co-transfected with or without p53 expressing plasmids, respectively, along with a β-galactosidase-expressing vector into human Saos-2 cells, followed by luciferase assay. The results are presented as fold activation ± standard error derived from three independent transfections, each run in duplicate, after normalization with β-galactosidase activity for transfection efficiency.