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. Author manuscript; available in PMC: 2009 Nov 27.
Published in final edited form as: J Phys Chem B. 2008 Nov 27;112(47):15034–15044. doi: 10.1021/jp805133u

Figure 4.

Figure 4

Laser flash photolysis (double photon excitation of water, 100 mM at 266 nm with detection at 330 nm) of N2O sat. solutions of 630 μM N-AcCysGly6 (grey squares in panels A and B) or mixtures of 630 μM N-Ac-Cys and 630 μM N-Ac-Gly-NH2 (grey diamonds in panels A and B). In the former cases, intramolecualr hydrogen transfer (equilibrium 6) is possible, whereas in the latter it is not. The filled circles in panels A and B represent the difference traces, so that the reactions in N-AcCysGly6 are corrected for all second order reactions. These differences represent intramolecular reactions. The kinetic isotope effect between solutions of H2O (panel A) and D2O (panel B) are clearly visible. Panel C directly compares the reactions of N-Ac-CysGly6 in D2O (grey dots, upper trace) with the ones in H2O (black dots, lower trace).