Figure 5. MetHb incubated over time at 21.9±0.1°C and 61.3±1.0 relative humidity ((▪) dry state, (•) hydrated state).

The samples were reacted with deoxyribose and then thiobarbituric acid to detect oxidative damage to the deoxyribose. The absorption measured at 528 nm is given after blank subtraction. Measurements are the average of three different samples incubated at the same time point.