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The sugar is degraded on exposure to hydroxyl radicals. The reaction mixure is heated under acidic conditions using TAA to form malondialdehyde (MDA) which reacts with TBA to form a pink chromogen. The absorption was measured at 532 nm after incubation of deoxyribose (▪), with 1.66 µg Fe(II) (•), and with 35 µg Hb (▴).
